穿孔素1(PRF1)重組蛋白
Recombinant Perforin 1 (PRF1)
FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
- 編號(hào)URPB317Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞膜, 分泌, 細(xì)胞質(zhì), 內(nèi)體
- 預(yù)測(cè)分子量63.4kDa
- 實(shí)際分子量62kDa(差異分析請(qǐng)參閱說(shuō)明書)
- 片段與標(biāo)簽Lys32~Phe316 with N-terminal His and GST Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)7.0
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見(jiàn)活性蛋白。 - 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 720 ¥ 1800 ¥ 3600 ¥ 10800 ¥ 27000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB317Hu02 | 穿孔素1(PRF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB317Hu01 | 穿孔素1(PRF1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB317Hu01 | 穿孔素1(PRF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB317Hu02 | 穿孔素1(PRF1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAB317Hu01 | 穿孔素1(PRF1)多克隆抗體 | IHC |
| ULAB317Hu81 | 穿孔素1(PRF1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAB317Hu71 | 穿孔素1(PRF1)多克隆抗體(生物素標(biāo)記) | IHC |
| UMAB317Hu22 | 穿孔素1(PRF1)單克隆抗體 | IHC |
| UMAB317Hu21 | 穿孔素1(PRF1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEB317Hu | 穿孔素1(PRF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB317Hu | 穿孔素1(PRF1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Diagnostic Microbiology and Infectious Disease | Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfection [Pubmed:26915636] |
| FASEB?JOURNAL | The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitis [Pubmed: 30951375] |
| Theranostics | A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors [Pubmed: 31695790] |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection [Pubmed: 33223223] |
| INVESTIGATIONAL NEW DRUGS | Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors [Pubmed: 32772342] |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237] |
| Nat Biomed Eng | Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy [34873307] |
| J Exp Med | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion [Pubmed:35061012] |
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