色素上皮衍生因子(PEDF)重組蛋白
Recombinant Pigment Epithelium Derived Factor (PEDF)
SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3
- 編號URPB972Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞膜
- 預測分子量58.2kDa
- 實際分子量58kDa(差異分析請參閱說明書)
- 片段與標簽Gln20~Thr418 with Two N-terminal Tags, His-tag and SUMO-tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點6.0
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 576 ¥ 1440 ¥ 2880 ¥ 8640 ¥ 21600
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產品包裝(模擬)
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產品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPB972Ra01 | 色素上皮衍生因子(PEDF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB972Ra01 | 色素上皮衍生因子(PEDF)多克隆抗體 | IHC |
| UMAB972Ra21 | 色素上皮衍生因子(PEDF)單克隆抗體 | WB; IHC; ICC; IP. |
| USEB972Ra | 色素上皮衍生因子(PEDF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB972Ra | 色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB972Ra | 色素上皮衍生因子(PEDF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB972Ra01 | 色素上皮衍生因子(PEDF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Molecular Therapy | A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions [Nature: 2010144] |
| American Journal of Nephrology | Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy [Karger: 314326] |
| 34 | Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury [OxfordJournals: source] |
| Heart and Education in Heart | The change of serum level of pigment epithelium-derived factor in coronary heart disease [Bmj: Source] |
| Mediators of Inflammation | Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation [Pubmed: 24288442] |
| Cardiology. | The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. [Pubmed: 24192856] |
| Endocrinology | Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism [Endocrine: Source] |
| Clinical Science | Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis [PubMed: 25700221] |
| Molecular Sciences | Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R [Pubmed:27187377] |
| Investigative ophthalmoloey & visual science | The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells [articleid:2582822] |
| Scientific reports | VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice [pubmed:28091556] |
| 國際眼科雜志 | 色素上皮衍生因子修飾的人臍帶間充質干細胞構建方法 [201707007] |
| Investigative?Ophthalmology?&?Visual?Science | PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice [Pubmed:30025136] |
| International Eye Science | 高海拔地區(qū)新生血管性青光眼不同治療方式的療效及對房水中PEDF, VEGF 水平的影響 [2018/2/201802020.pdf] |
| Domestic?Animal?Endocrinology | Lactation-related changes in tissue expression of PEDF in dairy cows [Pubmed:29758402] |
| Medical?Science?Monitor | Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a?… [Pubmed:29574467] |
| Chemosphere | Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis [Doi: 10.1016/j.chemosphere.2018.10.100] |
| Biological?Trace?Element?Research | Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model [Pubmed: 30392020] |
| International?Urology?and Nephrology | Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement [Pubmed: 30536192] |
| International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807] |
| Journal of the American Heart Association | Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia [Pubmed: 31711388] |
| environmental research | Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells [Pubmed: 32315827] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells [33498962] |
| Hum Cell | Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ?- [33768511] |
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