胱天蛋白酶3(CASP3)多克隆抗體
Polyclonal Antibody to Caspase 3 (CASP3)
CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1
- 編號UPAA626Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA626Hu02-胱天蛋白酶3(CASP3)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 283 ¥ 659 ¥ 942 ¥ 2355 ¥ 9420
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CASP3 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Thyroid Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human CASP3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human CASP3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human CASP3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant CASP3, Human.
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Western Blot; Sample: Lane1: Human Liver lysate; Lane2: Jurkat cell lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate; Lane5: Porcine Liver lysate; Lane6: Rat Cerebrum lysate<br/>Primary Ab: 0.5μg/ml Rabbit Anti-Human CASP3 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: CASP3 knockout Jurkat cell lysate;
Predicted MW: 31kDa
Observed MW: 31kDa
Primary Ab: 5μg/ml Rabbit Anti-Human CASP3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western?blot?shows?lysates?of?Jurkat?cell?line?treated?with?1?μM?staurosporine?(STS)?for?the?indicated?times.?
Primary?Ab:?0.5ug/ml?Rabbit?Anti-Human?CASP3?Antibody
Second?Ab:?0.2μg/mL?HRP-Linked?Caprine?Anti-Rabbit?IgG?Polyclonal?Antibody
(Catalog:?SAA544Rb19) -
Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Human peripheral blood mononuclear cell; Lane3: Porcine Liver lysate; Lane4: Rat Cerebrum lysate;<br/>Primary Ab: 0.2μg/ml Rabbit Anti-Human CASP3 Antibody<br/>Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對CASP3的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別CASP3。
用法
Western blotting: 0.01-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA626Hu01 | 胱天蛋白酶3(CASP3)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA626Hu01 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA626Hu02 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA626Hu03 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA626Hu03 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB |
| UPAA626Hu02 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB; IHC |
| UPAA626Hu01 | 胱天蛋白酶3(CASP3)多克隆抗體 | IHC |
| ULAA626Hu81 | 胱天蛋白酶3(CASP3)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA626Hu71 | 胱天蛋白酶3(CASP3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA626Hu21 | 胱天蛋白酶3(CASP3)單克隆抗體 | WB; IHC |
| UMAA626Hu22 | 胱天蛋白酶3(CASP3)單克隆抗體 | WB |
| USEA626Hu | 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA626Hu | 胱天蛋白酶3(CASP3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA626Hu01 | 胱天蛋白酶3(CASP3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
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| Molecules | Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway [Pubmed:27248986] |
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| Neurochemical?Research | Arecoline Induces Neurotoxicity to PC12 Cells: Involvement in ER Stress and Disturbance of Endogenous H2S Generation. [pubmed:27255601] |
| Molecular Medicine Reports | Hydrogen sulfide attenuates doxorubicin-induced cardiotoxicity by inhibiting the expression of peroxiredoxin III in H9c2 cells [mmr:13] |
| The American Journal of Chinese Medicine | Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells [pubmed:28231747] |
| bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. [pubmed:28187731] |
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| Environmental?Toxicology | Potential immunomodulatory and antioxidant effects of walnut ?vegetable oil against lead‐mediated hepatic damage and their interaction with lipase?… [Pubmed: 30251767] |
| Life?Sciences | Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096] |
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| Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats. [] | |
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| Marine Drugs | In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma [Pubmed: 32283782] |
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| Pharmaceutics | Chitosan-Based Microparticles Enhance Ellagic Acid's Colon Targeting and Proapoptotic Activity [Pubmed: 32660035] |
| Life Sciences | Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. [Pubmed: 32603817] |
| TOXICOLOGY | Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis [Pubmed: 32565124] |
| International Journal of Molecular Sciences | Optimized Conjugation of Fluvastatin to HIV-1 TAT Displays Enhanced Pro-Apoptotic Activity in HepG2 Cells [Pubmed: 32531976] |
| analytical chemistry | Fast Activation and Tracing of Caspase-3 Involved Cell Apoptosis by Combined Electrostimulation and Smart Signal-Amplified SERS Nanoprobes [Pubmed: 32395992] |
| Mar Drugs | Chitosan Coated Microparticles Enhance Simvastatin Colon Targeting and Pro-Apoptotic Activity [Pubmed: 32344610] |
| BIOMEDICINE & PHARMACOTHERAPY | Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939] |
| Iranian Journal of Basic Medical Sciences | Chromon-3-aldehyde derivatives restore mitochondrial function in rat cerebral ischemia [Pubmed: 32963739] |
| International Journal of Research in Pharmaceutical | In situ gel loaded with curcumin-based fluvastatin nanostructured carrier effective against squamous tongue carcinoma [] |
| SAGE Journals | Insights Into Protective Mechanisms of Dandelion Leaf Extract Against Cisplatin-Induced Nephrotoxicity in Rats: Role of Inhibitory Effect on Inflammatory and Apoptotic?… [] |
| Bangladesh Journal of Pharmacology | Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in rat [] |
| International Journal of Pharmacology | Ellagic Acid Loaded TPGS Micelles for Enhanced Anticancer Activities in Ovarian Cancer [] |
| Strawberry (Fragaria x ananassa) leaf extract reduces brain damage in diabetic male rats [] | |
| Indian Journal of Animal Research | Accacia senegel extract ameliorates Lead Acetate-mediated Hepatotoxicity in rats. [] |
| RESEARCH SQUARE | Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs [] |
| Egyptian Journal of Basic and Applied Sciences | Fish-oil supplementation improves retinal injury induced by diabetes and hypercholesterolemia in male Wistar rats [] |
| ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES [] | |
| Norwegian Journal of development of the International Science | CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER [] |
| J Biochem Mol Toxicol | Concanavalin A induces apoptosis in a dose?\dependent manner by modulating thiol/disulfide homeostasis in C6 glioblastoma cells [33604990] |
| International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis [33575317] |
| Sci Rep | Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats [33750916] |
| TOXICOLOGY IN VITRO | Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells [33675893] |
| Biomed Pharmacother | Captopril downregulates expression of Bax/cytochrome C/caspase-3 apoptotic pathway, reduces inflammation, and oxidative stress in cisplatin-induced acute??- [33945910] |
| Reprod Sci | Reproductive Effects of Nicotinamide on Testicular Function and Structure in Old Male Rats: Oxidative, Apoptotic, Hormonal, and Morphological Analyses [34101148] |
| BMC Complement Med Ther | Effect of the standard herbal preparation, STW5, treatment on dysbiosis induced by dextran sodium sulfate in experimental colitis [34103031] |
| Environ Sci Pollut Res Int | Comparative effects of metformin and Cistus laurifolius L. extract in streptozotocin-induced diabetic rat model: oxidative, inflammatory, apoptotic, and histopathological??- [34097215] |
| Piceatannol Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by Modulation of Nrf2/HO-1/NF|êB Axis. Front. Pharmacol. 11: 614897. doi??- [33519479] | |
| Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats [34426252] |
| Chem Biol Interact | Protective role of naringin loaded solid nanoparticles against aflatoxin B1 induced hepatocellular carcinoma [34717916] |
| Biochim Biophys Acta Gen Subj | Paraoxonase 2 protects against the CML mediated mitochondrial dysfunction through modulating JNK pathway in human retinal cells [34710487] |
| AAPS PharmSciTech | Development of an Icariin-Loaded Bilosome-Melittin Formulation with Improved Anticancer Activity against Cancerous Pancreatic Cells [34668089] |
| Analytical Cellular Pathology | The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia [34336554] |
| Ecotoxicol Environ Saf | Evaluation of cytogenotoxic potential and embryotoxicity of KRS-Cauvery River water in zebrafish (Danio rerio) [Pubmed:35183813] |
| Environ Sci Pollut Res Int | Chrysin attenuates estradiol-induced endometrial hyperplasia in rats via enhancing PPARα activity [Pubmed:35301625] |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats [Pubmed:35421180] |
| Journal of Ethnopharmacology | Ethnopharmacological impact of Melaleuca rugulosa (Link) Craven leaves extract on liver inflammation [Pubmed:35337921] |
| Eur J Pharmacol | Harmine prevents 3-nitropropionic acid-induced neurotoxicity in rats via enhancing NRF2-mediated signaling: Involvement of p21 and AMPK [Pubmed:35623405] |
| Gels | QbD Supported Optimization of the Alginate-Chitosan Nanoparticles of Simvastatin in Enhancing the Anti-Proliferative Activity against Tongue Carcinoma [Pubmed:35200484] |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?… [Pubmed:35715546] |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?… [Pubmed:35204140] |
| Value Health Sci | Oksidatif, Apoptotik ve ?nflamatuar Sinyal Yolaklar? üzerinden C6 Glioblastoma Hücrelerindeki ML351'in Antiproliferatif Etkileri [] |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?… [] | |
| Biointerface Research in Applied Chemistry | Promising Therapeutic Efficacy of Trigonella-foenum graecum and Bone Marrow-Derived Mesenchymal Stem Cells on Skeletal Muscle Atrophy in Experimental?… [] |
| Arab Journal of Nuclear Sciences and Applications | Potential activity of silver/hydroxyapatite nano composite against damaged liver consequence to irradiation [] |
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