磷脂酰肌醇蛋白聚糖4(GPC4)重組蛋白
Recombinant Glypican 4 (GPC4)
K-Glypican; Glypican Proteoglycan 4; Secreted glypican-4
- 編號URPA998Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞膜
- 預(yù)測分子量28.8kDa
- 實際分子量27kDa(差異分析請參閱說明書)
- 片段與標簽Ala222~Leu468 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點7.1
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 600 ¥ 1500 ¥ 3000 ¥ 9000 ¥ 22500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA998Ra01 | 磷脂酰肌醇蛋白聚糖4(GPC4)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA998Ra01 | 磷脂酰肌醇蛋白聚糖4(GPC4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA998Ra01 | 磷脂酰肌醇蛋白聚糖4(GPC4)多克隆抗體 | WB |
| USEA998Ra | 磷脂酰肌醇蛋白聚糖4(GPC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA998Ra | 磷脂酰肌醇蛋白聚糖4(GPC4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One. | Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation [Pubmed:Pmc4072672] |
| Aspects of fluid therapy in the critically ill. Experimental and clinical studies on fluid therapy in the inflammatory conditions. [250690_2_G5_Svajunas_S.pdf] | |
| Intensive?Care?Medicine?Experimental | Elevated plasma glypicans are associated with organ failure in patients with infection [Pubmed: 30618011] |
| Crit Care | Albumin infusion rate and plasma volume expansion: a randomized clinical trial in postoperative patients after major surgery [Pubmed: 31138247] |
| J Endocrinol Invest | Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … [Pubmed: 32816247] |
| Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study [] | |
| Sci Rep | Glypican-4 in pregnancy and its relation to glucose metabolism, insulin resistance and gestational diabetes mellitus status [34903856] |
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