降鈣素原(PCT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Procalcitonin (PCT)
Pro-Calcitonin
- 編號USEA689Ca
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.9pg/mL..
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1512 ¥ 2160 ¥ 9720 ¥ 18360 ¥ 151200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測降鈣素原(PCT)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的降鈣素原(PCT)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 92-101 | 97 |
| heparin plasma(n=5) | 91-98 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的降鈣素原(PCT)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中降鈣素原(PCT)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-89% | 83-91% | 85-94% | 94-104% |
| EDTA plasma(n=5) | 82-98% | 79-91% | 82-96% | 80-93% |
| heparin plasma(n=5) | 80-96% | 90-102% | 88-97% | 79-89% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將降鈣素原(PCT)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的降鈣素原(PCT)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的降鈣素原(PCT)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的降鈣素原(PCT)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA689Ca01 | 降鈣素原(PCT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA689Ca01 | 降鈣素原(PCT)多克隆抗體 | WB; IHC |
| USEA689Ca | 降鈣素原(PCT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA689Ca | 降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Scandinavian Journal of Infectious Diseases | Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis [PubMed: 20085423] |
| International Journal of Radiation Oncology ? Biology ? Physics | Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice [PubMed: 20637981] |
| Ulus Travma Acil Cerrahi Derg | Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia [PubMed: 21935794] |
| Egyptian Journal of Bronchology | Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children [Essbronchology: Source] |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis [Waikato:Source] |
| Egyptian Journal of Medical Microbiology | Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis [Ejmm:Source] |
| Nigerian Medical Journal | Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis [Pubmed:25657488] |
| Wiley online library | The use and value of procalcitonin in solid organ transplantation [PubMed: 25996831] |
| Balkan Med J | Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia [PubMed: 26185718] |
| The Journal of Clinical Endocrinology & Metabolism | Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay [PubMed: 26647152] |
| Journal of Human Hypertension | Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension [PubMed: 26040439] |
| Medical Science Monitor | Variation?of?Circulating?Inflammatory?Mediators?in?Staphylococcus?aureus?and?Escherichia?coli?BloodstreamInfection. [Pubmed:26772168] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
| Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005] |
| Acta?Medica | Risk Factors?of?Acute?Pancreatitis?in?Oral?Double Balloon Enteroscopy. [pubmed:27638962] |
| The Clinical Respiratory Journal | Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome [pubmed:28960823] |
| SHOCK | INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION [pubmed:27984536] |
| Cellular?Physiology and Biochemistry | Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure [Pubmed:29913443] |
| Archives of Medical Science | Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase [Pubmed:29593801] |
| Journal of Thoracic Disease | Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung [Pubmed:29707313] |
| Asian?Journal of Psychiatry | Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients [Pubmed:29723719] |
| European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341] |
| Medicina | Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection [Pubmed: 33080909] |
| MEDIATORS OF INFLAMMATION | Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase [Pubmed: 32410851] |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes [] | |
| Journal of Immunology Research | Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis [33628853] |
| Journal of Medical Biochemistry | Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer [33776564] |
| Surg Endosc | Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity [Pubmed:35044516] |
| Bone Res | Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment [Pubmed:35087025] |
