愛帕琳肽(APLN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Apelin (APLN)
XNPEP2; APEL; APJ endogenous ligand
- 編號UKSD066Ra11
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度4.84pg/mL
- 應(yīng)用Main materials for "Do It(ELISA Kit) Yourself"
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 4104 ¥ 6840 ¥ 12312 ¥ 23940 ¥ 41040
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對愛帕琳肽(APLN)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPD066Ra01 | 愛帕琳肽(APLN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAD066Ra01 | 愛帕琳肽(APLN)多克隆抗體 | IHC |
| ULAD066Ra71 | 愛帕琳肽(APLN)多克隆抗體(生物素標記) | IHC |
| UMAD066Ra21 | 愛帕琳肽(APLN)單克隆抗體 | WB; IHC; ICC; IP. |
| USED066Ra | 愛帕琳肽(APLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCED066Ra | 愛帕琳肽(APLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMD066Ra | 愛帕琳肽(APLN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSD066Ra11 | 愛帕琳肽(APLN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻
| 雜志 | 參考文獻 |
| Clin Exp Hypertens. | Individual and Concomitant Effects of Cardioprotective Programs on Cardiac Apelinergic System and Oxidative State in └-NAME-Induced Hypertension [Informahealthcare: Source] |
| African Journal of Pharmacy and Pharmacology | Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat. [Academicjournals: Source] |
| Zahedan Journal of research in Medical Sciences | Improvement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Hypertension Rats [Zjrms: Source] |
| Endocr J. | CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658] |
| Mol Metab | TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic mice [PubMed: 26500845] |
| Journal of medicinal food | Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design. [pubmed:28170279] |
| Arch Physiol Biochem | Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696] |
| preprints | Serum Apelin and Galactin-3 in Preeclampsia in Iraq [Pubmed: 32538210] |
| Journal of Diabetes Research | The Potential Role of Chemerin, Lipocalin 2, and Apelin in the Diagnosis and Pathophysiology of Gestational Diabetes Mellitus [] |
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