巨噬細(xì)胞移動(dòng)抑制因子(MIF)多克隆抗體
Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
- 編號UPAA698Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA698Mu01-巨噬細(xì)胞移動(dòng)抑制因子(MIF)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 292 ¥ 680 ¥ 972 ¥ 2430 ¥ 9720
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse MIF Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse MIF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Testis Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse MIF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: Hepa1-6 cell
Primary Ab: 20μg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant MIF, Mouse.
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Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Mastadenoma lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Jurkat cell lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Mouse MIF Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Mouse Kidney lysate; Lane2: Mouse Cerebrum lysate; Lane3: Jurkat cell lysate; Lane4: Hela cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對MIF的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別MIF。
用法
Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA698Mu01 | 巨噬細(xì)胞移動(dòng)抑制因子(MIF)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA698Mu02 | 巨噬細(xì)胞移動(dòng)抑制因子(MIF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA698Mu01 | 巨噬細(xì)胞移動(dòng)抑制因子(MIF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA698Mu01 | 巨噬細(xì)胞移動(dòng)抑制因子(MIF)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA698Mu02 | 巨噬細(xì)胞移動(dòng)抑制因子(MIF)多克隆抗體 | WB; IHC; ICC; IP. |
| UFAA698Mu01 | 抗巨噬細(xì)胞移動(dòng)抑制因子(MIF)多克隆抗體 | FCM |
| USEA698Mu | 巨噬細(xì)胞移動(dòng)抑制因子(MIF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA698Mu | 巨噬細(xì)胞移動(dòng)抑制因子(MIF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA698Mu | 巨噬細(xì)胞移動(dòng)抑制因子(MIF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Kidney International | Targeted reduction of advanced glycation improves renal function in obesity [PubMed: 21412218] |
| The Journal of Immunology | Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut [Jimmunol: 5673] |
| The Journal of Endocrinology | Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease [PubMed: PMC3757527] |
| The Journal of Immunology | Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice [PubMed: PMC3466372] |
| Diabetologia | Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes [Pubmed:24957662] |
| BioMed Research International | The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder [PubMed: 25705692] |
| Diabetologia | Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease. [pubmed:28160092] |
| Life Sciences | Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis [pubmed:28919396] |
| Scientific Reports | Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke [Pubmed:29335619] |
| Ocular?Immunology?and?Inflammation | Immune Response and Mechanisms of IFN-γ in Administration for Keratomycosis [Pubmed: 30307777] |
| International Journal of Immunopathology and Pharmacology | Effect of voluntary running on expression of myokines in brains of rats with depression [Pubmed: 30834799] |
| Biomarkers in Heart Failure and Associated Diseases | Biomarkers in Heart Failure and Associated Diseases [] |
| Science China-Life Sciences | Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation [Pubmed: 31209799] |
| Folia Morphologica | Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression [Pubmed: 31802473] |
| Biomed Pharmacother | Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis [Pubmed: 32062385] |
| Sci Rep | Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population [33850223] |
| Commun Biol | Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes [Pubmed:35115654] |
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