分泌型卷曲相關(guān)蛋白2(SFRP2)活性蛋白
Active Secreted Frizzled Related Protein 2 (SFRP2)
FRP-2; SARP1; SDF-5; Secreted apoptosis-related protein 1
- 編號UAPF879Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點7.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 暫無報價 暫無報價 暫無報價 暫無報價 暫無報價
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Secreted Frizzled-Related Protein 2 (SFRP2) is a key member of the SFRP family of extracellular signaling molecules. By inhibiting the Wnt/β-catenin pathway, SFRP2 plays a crucial role in regulating fundamental cellular processes. Its primary functions include the suppression of cellular proliferation and the induction of apoptosis. To test the effect of SFRP2 on cell proliferation, A549 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human SFRP2. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant human SFRP2 for 72h. The result was shown in Figure 1. It was obvious that SFRP2 significantly increased cell viability of A549 cells. The ED50 of recombinant human SFRP2 is 1.655 μg/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPF879Hu01 | 分泌型卷曲相關(guān)蛋白2(SFRP2)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPF879Hu01 | 分泌型卷曲相關(guān)蛋白2(SFRP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAF879Hu01 | 分泌型卷曲相關(guān)蛋白2(SFRP2)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAF879Hu21 | 分泌型卷曲相關(guān)蛋白2(SFRP2)單克隆抗體 | WB; IHC; ICC; IP. |
| USEF879Hu | 分泌型卷曲相關(guān)蛋白2(SFRP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMF879Hu | 分泌型卷曲相關(guān)蛋白2(SFRP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Experimental & Clinical Cancer Research | PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway [pubmed:28645312] |
| Biochemical genetics | Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer [pubmed:28421310] |
| Respiratory physiology & neurobiology | sFRP2 promotes airway inflammation and Th17/Treg imbalance in COPD via Wnt/β-catenin pathway [Pubmed: 31430541] |
| Stem Cell Research | Ocular Surface Ectoderm Instigated by WNT inhibition and BMP4 [Pubmed: 32603880] |
