頭蛋白(NOG)活性蛋白
Active Noggin (NOG)
SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)
- 編號UAPC130Ra02
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 624 ¥ 1560 ¥ 3120 ¥ 9360 ¥ 23400
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
Gene sequencing
-
Figure. SDS-PAGE; Sample: Active recombinant NOG, Rat.
-
Figure. Western Blot; Sample: Recombinant NOG, Rat.
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC130Ra01 | 頭蛋白(NOG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC130Ra01 | 頭蛋白(NOG)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPC130Ra02 | 頭蛋白(NOG)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC130Ra02 | 頭蛋白(NOG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC130Ra01 | 頭蛋白(NOG)多克隆抗體 | Testing in progress |
| UPAC130Ra02 | 頭蛋白(NOG)多克隆抗體 | IHC |
| USEC130Ra | 頭蛋白(NOG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC130Ra | 頭蛋白(NOG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSC130Ra01 | 頭蛋白(NOG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Arthritis Rheumatol | Imbalance between BMP2 and Noggin induces abnormal osteogenic differentiation of mesenchymal stem cells in ankylosing spondylitis [PubMed: 26413886] |
| Arthritis Rheumatol.? | Imbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal Osteogenic Differentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis [Pubmed:26413886] |
| Clin Exp Pharmacol Physiol. | Oxygen-glucose deprivation preconditioning protects neurons against oxygen-glucose deprivation/reperfusion induced injury via bone morphogenetic protein-7 mediated ERK, p38 and Smad signalling pathways. [Pubmed:26385023] |
| European cells & materials | The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. [pubmed:28266688] |
| Arthritis?&?Rheumatology | mbalance?Between?Bone?Morphogenetic?Protein?2?and?Noggin?Induces?Abnormal?OsteogenicDifferentiation?of?Mesenchymal?Stem Cells in Ankylosing Spondylitis. [pubmed:26413886] |
| Biotechnology and?Applied?Biochemistry | The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells. [pubmed:28981962] |
| Heliyon | Efficient in vitro delivery of Noggin siRNA enhances osteoblastogenesis [pubmed:29167826] |
| Journal of Cellular Biochemistry | Low‐intensity pulsed ultrasound promotes bone morphogenic protein 9‐induced osteogenesis and suppresses inhibitory effects of inflammatory cytokines on cellular?… [Pubmed: 31006911] |
| Aging Male | Effect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatment [Pubmed: 32160825] |
| J Reconstr Microsurg | Effect of Simvastatin Use in Free Tissue Transfer: An Experimental Study in a Rat Epigastric Free Flap Model [Pubmed: 31995818] |
| Biochem Biophys Rep | Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP [Pubmed:35005256] |
