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成纖維細(xì)胞生長(zhǎng)因子17(FGF17)活性蛋白

Active Fibroblast Growth Factor 17 (FGF17)

  • 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)活性蛋白 產(chǎn)品包裝(模擬)
  • 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)活性蛋白 產(chǎn)品包裝(模擬)
  • APC910Hu02.jpg Figure. SDS-PAGE
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Fibroblast?growth?factor?17 (FGF17) is?a?member?of?the?fibroblast?growth?factorfamily. FGF?family?members?possess?broad?mitogenicand?cell?survival?activities?and?are?involved?in?a?varietyof?biological?processes,?including?embryonicdevelopment?cell?growth,?morphogenesis,?tissue?repair,?tumor?growth,?andinvasion. Fibroblast Growth Factor Receptor 1 (FGFR1) is high affinity receptor for FGF17, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGF17 and recombinant human FGFR1. Briefly, biotin-linked FGF17 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF17 and human FGFR1 was shown in Figure 1, the EC50?for this effect is 0.52 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
URPC910Hu01 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPC910Hu02 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)活性蛋白 Cell?culture;?Activity?Assays.
URPC910Hu02 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPC910Hu01 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)活性蛋白 Cell?culture;?Activity?Assays.
UPAC910Hu01 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)多克隆抗體 WB; IHC; ICC; IP.
UMAC910Hu21 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)單克隆抗體 WB; IHC; ICC; IP.
USEC910Hu 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMC910Hu 成纖維細(xì)胞生長(zhǎng)因子17(FGF17)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
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