粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)活性蛋白
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor
- 編號(hào)UAPA045Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)5.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 648 ¥ 1620 ¥ 3240 ¥ 9720 ¥ 24300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: APA045Hu61; Antibody: PAA045Hu06
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
TF-1, the human erythroleukemia cell line,provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. In house data was obtained by the following experiment: TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope everyday . <br/>Cell proliferation of TF1 cells after incubation with GM-CSF (10ng/mL) for 3 days was shown in Figure 1.The ED50 is 0.74ng/ml.
Cell differentiation of U937 cells after incubation with GM-CSF (10ng/mL) for 5 days was shown in Figure 2.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA045Hu61 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA045Hu61 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA045Hu01 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA045Hu01 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA045Hu01 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)多克隆抗體 | IHC |
| UPAA045Hu06 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)多克隆抗體 | WB; IHC |
| UFAA045Hu31 | 抗粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)多克隆抗體(別藻藍(lán)蛋白/花青染料cy7標(biāo)記) | FCM |
| UMAA045Hu22 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)單克隆抗體 | WB; IHC; ICC; IP. |
| URAA045Hu21 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)重組抗體 | FCM |
| USEA045Hu | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA045Hu | 抗粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(Anti-GM-CSF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCA045Hu | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA045Hu | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA045Hu01 | 粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Toxicology and Environmental Health | Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic System [PubMed: 20391130] |
| 5 | Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trial [Pubmed:24525316] |
| Archives of Virology | Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China [Pubmed:29305646] |
| BioMed Research International | Activated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, Whereas Kupffer Cells Predominantly Express Anti?… [] |
| bulletin of experimental biology and medicine | Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin [Pubmed: 31183654] |
| Exp Mol Pathol | The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal??- [33285209] |
| Vaccine | Co-delivery of PSMA antigen epitope and mGM-CSF with a cholera toxin-like chimeric protein suppressed prostate tumor growth via activating dendritic cells and??- [33612342] |
